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Close all running programs. The more programs you re running, the less memory is available to other programs. Installers also typically update files and programs; if you keep other programs running installation may not work properly. Reference the ReadMe.txt: Please refer to the ReadMe file located at the root of the CD-ROM for the latest product information at the time of publication. If you still have trouble with the CD, please call the Wiley Publishing Customer Care phone number: (800) 762-2974. Outside the United States, call 1 (317) 572-3993. You can also contact Wiley Publishing Customer Service by e-mail at techsupdum@ wiley.com. Wiley Publishing will provide technical support only for installation and other general quality control items; for technical support on the applications themselves, consult the program s vendor or author.

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In this and their following different forms, the coef cient is the probability that the molecule colliding with the surface is neither immediately re ected nor has an immediate chemical reaction. This statement agrees with that of P schl et al. [4, p. 2130], and implies adsorption o at the surface, but not necessarily in a single-molecule-surface layer as in P schl et al. [4]. This o distinction is meaningful only if surface molecules are different from interior molecules, and if transfers between the surface layer and interior are treated separately. The coef cient is often denoted by M . Alternative similar de nitions are as follows: Surface accommodation coef cient. The name given by P schl et al. [4] for adsorption in the o surface layer, and denoted by SXi for a molecule Xi. Condensation coef cient. The probability of no immediate re ection and usually refers to condensation of a single substance. Widely used in chemical engineering literature and by Pruppacher and Klett [49]. Denoted by C . There is a corresponding evaporation coef cient, E , for a condensable molecule which appears in the outgoing ux from a surface, and which, by detailed balance, is equal to C at the same temperature.

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acid [Dunkelman et al, 1995; Vunjak-Novakovic et al, 1999], or natural, such as those derived from or analogous to collagen [Wakitani et al, 1989], hyaluronan (HA) [Robinson et al, 1990; Solchaga et al, 2001], brin [Itay et al, 1987; Hendrickson et al, 1994], and alginate- brin mixture [Perka et al, 2000] Other materials, such as agarose, have been used primarily as model systems for analyzing the regulation of tissue formation [Buschmann et al, 1992, 1995; Mauck et al, 2003] Other methods have been developed to form implants that are composed only of cells and their products Immature chondrocytes can form cartilaginous tissue when cultured as a monolayer at high density in the absence of a scaffold [Adkisson et al, 2001] or as a multilayer [Yu et al.

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Go to " Add -Ins" tab to activate "KA.Barcode for Excel " setting panel. Choose a list of cells, select " DataMatrix " and enter your valid data . Alternatively, select a list of cells with required data , and choose " DataMatrix " barcode type. Then click " Insert " to generate the Data Matrix barcode image list in Excel .
Go to " Add -Ins" tab to activate "KA.Barcode for Excel " setting panel. Choose a list of cells, select " DataMatrix " and enter your valid data . Alternatively, select a list of cells with required data , and choose " DataMatrix " barcode type. Then click " Insert " to generate the Data Matrix barcode image list in Excel .

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Cell culture asks Centrifuge Centrifuge tubes CMF-PBS Collagenase powder Conical centrifuge tube Cryogenic vial Culture bags Dimethyl sulfoxide (DMSO) Dissection microscope with 0.7 1.0 magni cation DNase II Dulbecco s modi ed Eagle s medium (DMEM) DMEM high glucose (Lelkes and Unsworth) Dulbecco s modi ed minimal Eagle s medium- Ham s F-12 nutrient mixture F12H medium Fetal bovine serum Fetal bovine serum Fetal bovine serum Fiber optic illumination Flasks (T175) Forceps #55 Gas-permeable tissue culture bags Gentamycin sulfate German glass coverslips

To edit a group or an object within a group:

1997], a con guration that recapitulates the high density of cells in fetal cartilage [Li et al, 2001] On the other hand, chondrocytes from adult articular cartilage form only a very small amount of matrix when cultured under the same conditions [Adkisson et al, 2001] For the production of cartilaginous tissue in vitro, we developed a two-step culture method, termed the alginate-recovered chondrocyte (ARC) method [Masuda et al, 2003] The ARC method allows formation of cartilaginous tissue from animal or human cells with culture in alginate gel as an intermediate step The methodology is robust enough to use cells from adults, which are typically dif cult to stabilize in a chondrogenic phenotype The rst step of the ARC method consists of culturing chondrocytes encapsulated in alginate gel under conditions that maintain the normal phenotype of chondrocytes and modulate the formation of a CM rich in aggrecan molecules.

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Culture of Cells for Tissue Engineering, edited by Gordana Vunjak-Novakovic and R. Ian Freshney Copyright 2006 John Wiley & Sons, Inc.

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