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After 1 week, the alginate gel is solubilized and the cells with their attached CM are recovered and cultured on a porous membrane Within 1 further week the alginate-recovered chondrocytes become integrated into a cohesive cartilaginous tissue mass containing abundant amounts of aggrecan and type II collagen and minimal amounts of type I collagen [Masuda et al, 2003] Once there is a suf cient quantity of cell-associated matrix in the cultured alginate beads, the beads can be dissolved and the cells with their cell-associated matrix recovered For bovine cells, an appropriate preculture period is 7 10 days when cultured in the presence of 20% FBS For human cells, approximately 2 weeks is effective, when incubation is done in medium supplemented with 200 ng/ml osteogenic protein 1 (OP-1; BMP-7) and 20% FBS..

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Theorem 10.5 (Connectivity of DLSS [37]): DLSS preserves the connectivity of G, that is, DLSS is strongly connected if G is strongly connected. Proof. Suppose G is strongly connected. For any two nodes u, v [ V(G), there exists at least one path p (w0 u, w1, w2, . . . , wm21, wm v) from u to v,

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m1 X j 1 m1 h i X z k 1 z k 1 , z k 1 exp g 2 k 1 , where s k 1 b1j k 1 , b k 1 j 1 1j 1j 1j n h 1 i X j 1 w1ij xi k 1 c , v , w , and c are the optimal parameters that g 1j k 1 1ij 1ij 1ij 1ij i 1 v s k 1 2fi;k 1 V1i s k 1 2fi;k 1 1ij j

8841 Resiliency of 2PC and Failure Handling The 2PC protocol is more resilient than the 1PC since it has a much smaller WoV The WoV in the 2PC is much smaller than the one in 1PC, since it does not include the transaction execution time Prior to entering the Prepared state, if the coordinator fails, slaves can abort the transaction by voting Not Ready This protocol, however, is not capable of dealing with the coordinator s failure in the second phase of the protocol It should be easy to see that the only time when the slaves are blocked from making a unilateral decision to abort a transaction is when they have entered their Prepared states To deal with failures, the 2PC must be extended to include a termination protocol and a recovery protocol.

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By using the logical AND operator (&), you mask off all the other bits to leave only the one you want. In practice you don t usually do the left-shift of a bit position but just use the numeric value it represents. For example, a bit in position 2 has a value of 0x04, because (2<<1) == 0x04. You can remember these bit masks by making them usefully named constants, for example BUMPLEFT_MASK to get the bump left bit. To help in remembering which byte of the array to work on, also define constants for the positions in the array, like BUMPWHEELDROPS for the first byte in the array. These constant definitions aren t necessary, but they do cut down on the typos. The preceding equation is actually a little wrong, especially for Java. The masking returns the byte value of the bit set. So if it were the left-most bit, the value after masking would be either zero or 127 (0x80). This can lead to all sorts of nasty, hard-to-find bugs. Java tries to help you out with this by forcing you to convert a number to a Boolean value. In Java, the preceding would really be written as:

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These protocols together guide sites through a set of steps to deal with failures The termination protocol is intended to terminate a transaction when one or more sites fail This protocol is invoked by all operational sites after a failure of a site is detected The termination protocol tries to commit or abort the transaction after a failure as soon as possible to release data resources locked by the sites involved in the transaction The recovery protocol is run by the sites that have failed after they are repaired A failed site is not involved in the decision that is made about termination of the transaction and, therefore, it must terminate the transaction exactly as others have We assume the inclusion of a timeout mechanism that allows the coordinator to detect the failures of the slaves and the slaves to detect the coordinator s failure.

The four sizes of virtual tributary frames. (From Ref. 2, courtesy of Hewlett-Packard.)

2.2.2.1 Primary Horizontal Fragmentation Primary horizontal fragmentation (PHF) partitions a table horizontally based on the values of one or more columns of the table. Example 2.2 discusses the creation of three PHF fragments from the EMP table based on the values of the Loc column.

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